Journal: Scientific Reports

Article Title: Exploring the identification of multiple bacteria on stainless steel using multi-scale spectral imaging from microscopic to macroscopic

doi: 10.1038/s41598-022-19617-3

Figure Lengend Snippet: (Top panel): Mean FTIR spectra (after applying asymmetric least squares smoothing to remove baseline) of each bacterial species. The offset is manually added for better visualisation. (Bottom panel): Resultant FTIR spectra after applying the second derivative (window size = 15 points and the polynomial order = 3) on the mean spectrum of each bacterial species. R = Reflectance. Arb.units = Arbitrary units. BS: Bacillus subtilis ; LP: Lactobacillus plantarum ; EC: Escherichia coli , CS: Cronobacter sakazakii ; PF: Pseudomonas fluorescens.

Article Snippet: The bacteria species used in this study, representing a selection of GP and GN bacteria, were: Bacillus subtilis (B. subtilis) DSM 10, Lactobacillus plantarum (L. plantarum) DSM 20174, Escherichia coli (E. coli) DSM 11250, Cronobacter sakazakii (C. sakazakii) ATCC 29544 , and Pseudomonas fluorescens (P. fluorescens) DSM 50090, respectively labelled as BS, LP, EC, CS and PF for the sake of simplicity.

Techniques:

Journal: Scientific Reports

Article Title: Exploring the identification of multiple bacteria on stainless steel using multi-scale spectral imaging from microscopic to macroscopic

doi: 10.1038/s41598-022-19617-3

Figure Lengend Snippet: Mean VNIR ( A ) and SWIR ( B ) spectra of all bacterial species collected from stainless steel. R = Reflectance. Arb.units = Arbitrary units. BS: Bacillus subtilis ; LP: Lactobacillus plantarum ; EC: Escherichia coli , CS: Cronobacter sakazakii ; PF: Pseudomonas fluorescens.

Article Snippet: The bacteria species used in this study, representing a selection of GP and GN bacteria, were: Bacillus subtilis (B. subtilis) DSM 10, Lactobacillus plantarum (L. plantarum) DSM 20174, Escherichia coli (E. coli) DSM 11250, Cronobacter sakazakii (C. sakazakii) ATCC 29544 , and Pseudomonas fluorescens (P. fluorescens) DSM 50090, respectively labelled as BS, LP, EC, CS and PF for the sake of simplicity.

Techniques:

Journal: Scientific Reports

Article Title: Exploring the identification of multiple bacteria on stainless steel using multi-scale spectral imaging from microscopic to macroscopic

doi: 10.1038/s41598-022-19617-3

Figure Lengend Snippet: Score and loading plots (PC1 and PC2) of the PCA model built from the calibration sets of mean spectra for each modality. Note: A circle marker refers to the observation in the training set, while a diamond marker denotes the sample in the validation or test set. BS: Bacillus subtilis ; LP: Lactobacillus plantarum ; EC: Escherichia coli , CS: Cronobacter sakazakii ; PF: Pseudomonas fluorescens.

Article Snippet: The bacteria species used in this study, representing a selection of GP and GN bacteria, were: Bacillus subtilis (B. subtilis) DSM 10, Lactobacillus plantarum (L. plantarum) DSM 20174, Escherichia coli (E. coli) DSM 11250, Cronobacter sakazakii (C. sakazakii) ATCC 29544 , and Pseudomonas fluorescens (P. fluorescens) DSM 50090, respectively labelled as BS, LP, EC, CS and PF for the sake of simplicity.

Techniques: Marker, Biomarker Discovery

Journal: Scientific Reports

Article Title: Exploring the identification of multiple bacteria on stainless steel using multi-scale spectral imaging from microscopic to macroscopic

doi: 10.1038/s41598-022-19617-3

Figure Lengend Snippet: Classification maps for samples in the test sets obtained from the best mean (top panel) and pixel-level (bottom panel) FTIR models for GP/GN classification. Pixels classified as GP/GN are shown in blue or orange respectively. Each replicate has two drops. BS: Bacillus subtilis ; LP: Lactobacillus plantarum ; EC: Escherichia coli , CS: Cronobacter sakazakii ; PF: Pseudomonas fluorescens. White scalebar indicates 1 mm (width) × 0.2 mm (height).

Article Snippet: The bacteria species used in this study, representing a selection of GP and GN bacteria, were: Bacillus subtilis (B. subtilis) DSM 10, Lactobacillus plantarum (L. plantarum) DSM 20174, Escherichia coli (E. coli) DSM 11250, Cronobacter sakazakii (C. sakazakii) ATCC 29544 , and Pseudomonas fluorescens (P. fluorescens) DSM 50090, respectively labelled as BS, LP, EC, CS and PF for the sake of simplicity.

Techniques:

Journal: Scientific Reports

Article Title: Exploring the identification of multiple bacteria on stainless steel using multi-scale spectral imaging from microscopic to macroscopic

doi: 10.1038/s41598-022-19617-3

Figure Lengend Snippet: Classification maps for samples in the test set (reps 6, 9, 12, and 16) obtained from the best mean and pixel-level VNIR/SWIR models for GP/GN classification. Pixels classified as GP/GN are shown in blue or orange respectively. Each replicate has two drops. BS: Bacillus subtilis ; LP: Lactobacillus plantarum ; EC: Escherichia coli , CS: Cronobacter sakazakii ; PF: Pseudomonas fluorescens. White scalebar indicates 1 mm (width) × 0.25 mm (height).

Article Snippet: The bacteria species used in this study, representing a selection of GP and GN bacteria, were: Bacillus subtilis (B. subtilis) DSM 10, Lactobacillus plantarum (L. plantarum) DSM 20174, Escherichia coli (E. coli) DSM 11250, Cronobacter sakazakii (C. sakazakii) ATCC 29544 , and Pseudomonas fluorescens (P. fluorescens) DSM 50090, respectively labelled as BS, LP, EC, CS and PF for the sake of simplicity.

Techniques:

Journal: Scientific Reports

Article Title: Exploring the identification of multiple bacteria on stainless steel using multi-scale spectral imaging from microscopic to macroscopic

doi: 10.1038/s41598-022-19617-3

Figure Lengend Snippet: Classification maps for samples in the test set (reps 6, 9, 12, and 16) obtained from the best pixel-level FTIR models for GN EC/CS/PF classification (pixels classified as EC/CS/PF are shown in orange, blue or magenta respectively). Each replicate has two drops. EC: Escherichia coli , CS: Cronobacter sakazakii ; PF: Pseudomonas fluorescens. White scalebar indicates 1 mm (width) × 0.2 mm (height).

Article Snippet: The bacteria species used in this study, representing a selection of GP and GN bacteria, were: Bacillus subtilis (B. subtilis) DSM 10, Lactobacillus plantarum (L. plantarum) DSM 20174, Escherichia coli (E. coli) DSM 11250, Cronobacter sakazakii (C. sakazakii) ATCC 29544 , and Pseudomonas fluorescens (P. fluorescens) DSM 50090, respectively labelled as BS, LP, EC, CS and PF for the sake of simplicity.

Techniques:

Journal: Scientific Reports

Article Title: Exploring the identification of multiple bacteria on stainless steel using multi-scale spectral imaging from microscopic to macroscopic

doi: 10.1038/s41598-022-19617-3

Figure Lengend Snippet: Classification maps for samples in the test set (reps 6, 9, 12, and 16) obtained from the best pixel level VNIR/SWIR models for GN species EC/CS/PF classification (pixels classified as EC/CS/PF are shown in orange, blue or magenta respectively). Each replicate has two drops. EC: Escherichia coli , CS: Cronobacter sakazakii ; PF: Pseudomonas fluorescens. White scalebar indicates 1 mm (width) × 0.2 mm (height).

Article Snippet: The bacteria species used in this study, representing a selection of GP and GN bacteria, were: Bacillus subtilis (B. subtilis) DSM 10, Lactobacillus plantarum (L. plantarum) DSM 20174, Escherichia coli (E. coli) DSM 11250, Cronobacter sakazakii (C. sakazakii) ATCC 29544 , and Pseudomonas fluorescens (P. fluorescens) DSM 50090, respectively labelled as BS, LP, EC, CS and PF for the sake of simplicity.

Techniques:

Journal: Scientific Reports

Article Title: Exploring the identification of multiple bacteria on stainless steel using multi-scale spectral imaging from microscopic to macroscopic

doi: 10.1038/s41598-022-19617-3

Figure Lengend Snippet: Classification maps for samples in the test set (reps 6, 9, 12, and 16) obtained from the best pixel-level PCA-LSTM VNIR/SWIR model for GP/GN classification. Pixels classified as GP/GN are shown in blue or orange respectively. Each replicate has two drops. BS: Bacillus subtilis ; LP: Lactobacillus plantarum ; EC: Escherichia coli , CS: Cronobacter sakazakii ; PF: Pseudomonas fluorescens.

Article Snippet: The bacteria species used in this study, representing a selection of GP and GN bacteria, were: Bacillus subtilis (B. subtilis) DSM 10, Lactobacillus plantarum (L. plantarum) DSM 20174, Escherichia coli (E. coli) DSM 11250, Cronobacter sakazakii (C. sakazakii) ATCC 29544 , and Pseudomonas fluorescens (P. fluorescens) DSM 50090, respectively labelled as BS, LP, EC, CS and PF for the sake of simplicity.

Techniques:

Journal:

Article Title: Effects of Iron Limitation on the Degradation of Toluene by Pseudomonas Strains Carrying the TOL (pWWO) Plasmid

doi: 10.1128/AEM.67.8.3406-3412.2001

Figure Lengend Snippet: Effects of iron/toluene ratio on substrate removal and yield for P. putida mt2 (A) and P. putida WCS358(pWWO) (B). Cell cultures were grown on mineral medium in a chemostat at a fixed dilution rate of 0.1 h−1. The toluene concentration was kept constant, while the iron concentration was varied. The yield (expressed in grams [dry weight] of cells [cdw] per gram of toluene consumed) (■), residual iron concentration (▴), and residual toluene concentration (○) were determined.

Article Snippet: P. putida mt2 (= ATCC 33015) harbors the TOL plasmid pWWO ( 3 ).

Techniques: Concentration Assay

Journal:

Article Title: Effects of Iron Limitation on the Degradation of Toluene by Pseudomonas Strains Carrying the TOL (pWWO) Plasmid

doi: 10.1128/AEM.67.8.3406-3412.2001

Figure Lengend Snippet: Activities of the TOL pathway enzymes in continuous cultures of P. putida mt2 (●) and P. putida WCS358(pWWO) (○) grown on toluene. The toluene monooxygenase (TMO) (A) and benzoate-1,2-dioxygenase (B12O) (C) activities are described by the rate of oxygen consumption (micromoles per minute per gram [dry weight] of cells, equivalent to units per gram [dry weight] of cells). The benzyl alcohol dehydrogenase (BADH) (B), catechol-2,3-dioxygenase (C23O) (D), and HMSH (E) activities are described by the rate of substrate conversion (micromoles per minute per gram of protein in cell extract, equivalent to units per gram of protein in cell extract). cdw, dry weight of cells.

Article Snippet: P. putida mt2 (= ATCC 33015) harbors the TOL plasmid pWWO ( 3 ).

Techniques:

Journal:

Article Title: Effects of Iron Limitation on the Degradation of Toluene by Pseudomonas Strains Carrying the TOL (pWWO) Plasmid

doi: 10.1128/AEM.67.8.3406-3412.2001

Figure Lengend Snippet: Kinetic parameters for toluene degradation by P. putida mt2 cells grown in continuous culture with different iron/toluene ratios a

Article Snippet: P. putida mt2 (= ATCC 33015) harbors the TOL plasmid pWWO ( 3 ).

Techniques:

Journal:

Article Title: Effects of Iron Limitation on the Degradation of Toluene by Pseudomonas Strains Carrying the TOL (pWWO) Plasmid

doi: 10.1128/AEM.67.8.3406-3412.2001

Figure Lengend Snippet: Total biomass (expressed in grams [dry weight] of cells [cdw] per liter) formed during growth of P. putida mt2 in continuous culture on medium containing 15 mM citrate amended with 10, 5, or 1 μM FeCl3. The TOL pathway enzymes were either not induced or induced by the gratuitous inducer o-xylene.

Article Snippet: P. putida mt2 (= ATCC 33015) harbors the TOL plasmid pWWO ( 3 ).

Techniques:

Journal:

Article Title: Effects of Iron Limitation on the Degradation of Toluene by Pseudomonas Strains Carrying the TOL (pWWO) Plasmid

doi: 10.1128/AEM.67.8.3406-3412.2001

Figure Lengend Snippet: Effects of carbon source on iron utilization by P. putida mt2 and P. putida WCS358(pWWO) under iron-excess (10 μM FeCl 3 ) and iron-limited (1 μM FeCl 3 ) conditions

Article Snippet: P. putida mt2 (= ATCC 33015) harbors the TOL plasmid pWWO ( 3 ).

Techniques:

Journal:

Article Title: Effects of Iron Limitation on the Degradation of Toluene by Pseudomonas Strains Carrying the TOL (pWWO) Plasmid

doi: 10.1128/AEM.67.8.3406-3412.2001

Figure Lengend Snippet: Effects of iron concentration on the activities of the TOL pathway enzymes in continuous cultures of P. putida mt2 growing on 15 mM citrate

Article Snippet: P. putida mt2 (= ATCC 33015) harbors the TOL plasmid pWWO ( 3 ).

Techniques: Concentration Assay